Appeal No. 2000-1930 Page 2
Application No. 08/232,452
29. Transduced primary fibroblasts contained in a collagen matrix
suitable for implantation into the loose connective tissue of the
dermis of a subject, wherein said transduced fibroblasts are
infected with a recombinant retroviral vector that contains
exogenous genetic material encoding a gene product, wherein said
transduced fibroblasts express said gene product, and wherein
expression of said gene product is under the control of a
constitutive promoter.
30. A method for immunizing a subject against immunogenic,
exogenous material, said method comprising:
implanting in the loose connective tissue of the dermis of a
subject, an extracellular collagen matrix containing transduced
subject-derived primary fibroblasts, wherein said transduced
fibroblasts are infected with a recombinant retroviral vector
containing exogenous genetic material encoding an immunogenic
gene product, and wherein said transduced fibroblasts express
said gene product.
The examiner relies upon the following references:
Bell et al. (Bell), ”The reconstitution of living skin,” The Journal of Investigative
Dermatology, Vol. 81, No. 1, pp. 2s-10s (1983)
Miller et al. (Miller), ”Transfer of genes into human somatic cells using retrovirus
vectors,” Cold Spring Harbor Symposia on Quantitative Biology, Vol. LI,
pp.1013-1019 (1986)
Garver Jr., et al. (Garver I), ”Production of glycosylated physiologically ‘normal’
human α1-antitrypsin by mouse fibroblasts modified by insertion of a human
α1-antitrypsin cDNA using a retroviral vector,” Proc. Natl. Acad. Sci. USA, Vol.
84, pp.1050-1054 (1987)
Palmer et al. (Palmer), ”Efficient retrovirus-mediated transfer and expression of a
human adenosine deaminase gene in diploid skin fibroblasts from an adenosine
deaminase-deficient human,” Proc. Natl. Acad. Sci. USA, Vol. 84, pp. 1055-1059
(1987)
Selden et al. (Selden), ”Implantation of genetically engineered fibroblasts into
mice: Implications for gene therapy,” Science, Vol. 236, pp. 714-718 (1987)
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