Appeal No. 2001-2337
Application No. 07/493,442
Claim 9 is representative of the subject matter on appeal:
9. A dry multilayer analytical element for the determination of acetaminophen
in an aqueous fluid, comprising a support having thereon, in order from
said support and in fluid contact:
(a) a layer having therein (i) an arylacylamidase enzyme; (ii) a ferricyanide
capable of oxidatively coupling paraaminophenol to a color-forming
coupling agent to form a color compound; and (iii) a water-soluble,
color-forming coupling agent, at least one layer comprising gelatin and
said water-soluble, color-forming coupling agent having the general
formula:
wherein R is a water-solubilizing group selected from (1) – (CH2) nX,
where n is 1 to 5, X is either (I) –SO3M where M is hydrogen, an alkali
metal, an alkaline earth metal or an ammonium (NH4+) cation, or (II)
(-OCH2CH2) yOH where y is 2 to 5; and (2) – N(R7)3+Z- where each R7 is
independently selected from alkyl of 1 to 4 carbon atoms, and Z is an acid
anion;
R1 and R6 are taken together to represent an ethylene, trimethylene, or
tetramethylene group which forms a partially saturated ring; and
R2, R3, and R4 are independently selected from hydrogen, alkyl of 1 to 4
carbon atoms, and alkoxy of 1 to 4 carbon atoms;
(b) a porous spreading layer; and
(c) a buffer which maintains the pH of the element in a range of between
about 6.5 to 8.5.
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