Appeal No. 1997-2745 Page 2
Application No. 08/212,175
18. A process for removing wax from a wax-embedded biological tissue specimen,
said process comprising contacting said wax-embedded tissue specimen with a
dewaxing composition comprising a paraffin-solubilizing organic solvent selected from
the group consisting of aromatic hydrocarbons, terpenes and isoparaffinic
hydrocarbons, a polar organic solvent, and a surfactant to solubilize the wax associated
with the specimen.
20. A dewaxing kit for removing wax from a wax-embedded biological tissue
specimen comprising:
a receptacle adapted to hold individual reagent containers,
a first container containing a dewaxing composition comprising
a) a paraffin-solubilizing organic solvent selected from the group consisting of
aromatic hydrocarbons, terpenes and isoparaffinic hydrocarbons, comprising
from about 25% to about 75% by volume of said composition;
b) a polar organic solvent comprising from about 25% to about 75% by volume
of said composition; and
c) a surfactant comprising from about 0.5% to about 20% by weight to volume of
said composition;
to solubilize the wax in the specimen, and
a second container containing (1) an immunostaining reagent or (2) an aqueous
wash solution comprising a detergent for removing residual dewaxing composition from
said tissue specimen.
21. A composition for removing wax from a wax-embedded biological tissue
specimen comprising:
(a) at least one paraffin-solubilizing organic solvent selected from the group
consisting of aromatic hydrocarbons, terpenes and isoparaffinic hydrocarbons, said
paraffinic-solubilizing organic solvent comprising from about 25% to about 75% by
volume of said composition;
(b) at least one water soluble polar organic solvent, said water soluble polar
organic solvent comprising from about 25% to about 75% by volume of said
composition;
(c) at least one water soluble surfactant, said water soluble surfactant
comprising from about 0.5% to about 20% by weight to volume of said composition;
which composition removes wax from the tissue specimen.
THE REFERENCES
The prior art references relied on by the examiner are:
Gipp 4,530,781 Jul. 23, 1985
Stevens 5,124,062 Jun. 23, 1992
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