Appeal No. 1999-1355 Page 2
Application No. 08/469,786
Claims 35 and 42 are representative of the subject matter on appeal and read as
follows:
35. A chimeric antibody comprising an Ig moiety having antigen binding activity
and a non-Ig protein moiety comprising a protein having biological activity or a
biologically active portion thereof, wherein said non-Ig protein moiety is carboxy
terminal to said Ig moiety, said chimeric antibody having said antigen binding activity
and said biological activity.
42. A process for the production of a chimeric antibody comprising an Ig moiety
having antigen binding activity and an non-Ig protein moiety comprising a protein having
biological activity or a biologically active portion thereof, wherein said non-Ig protein
moiety is carboxy terminal to said Ig moiety, said chimeric antibody having said antigen
binding activity and said biological activity, said process comprising;
i) preparing a replicable expression vector comprising a promoter operably linked
to a DNA sequence comprising a first part encoding at least a variable region of an
antigen-binding Ig polypeptide chain and a second part 3' of said first part encoding a
biologically functional non-Ig protein, or a biologically active portion thereof, wherein
said first and second parts are combined such that expression results in a product
possessing said variable region capable of binding antigen and said non-Ig protein
capable of exhibiting its biological function as expressed;
ii) transforming an immortalized mammalian cell line that secretes an Ig
polypeptide chain complementary to the variable region encoded in said DNA sequence
with said vector; and
iii) culturing the transformed cell line under conditions such that said DNA
sequence is expressed and such that assembly of said chimeric antibody is effected so
that said variable region is immunologically active and said non-Ig protein moiety is
biologically functional.
The references relied on by the examiner are:
Hopp et al. (Hopp) 4,703,004 Oct. 27, 1987
Cabilly et al. (Cabilly) 4,816,567 Mar. 28, 1989
Lehninger, in Biochemistry, Worth Publishers, Inc., New York, NY, page 125 (1970)
Neuberger, “Expression and Regulation of Immunoglobulin Heavy Chain Gene
Transfected into Lymphoid Cells,” The EMBO Journal, Vol. 2, No. 8, pp. 1373-1378
(1983)
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