Appeal No. 2002-1630 Page 2
Application No. 09/175,713
5. A composition comprising an isolated polynucleotide encoding an
amino-terminal-modified chemokine, wherein the
amino-terminal-modified chemokine comprises at least one methionine
at least one aminooxypentane residue or at least one GroHEK peptide
covalently attached to the amino terminus of the chemokine, and
wherein the amino-terminal-modified chemokine is derived from a
chemokine selected from the group consisting of SDF-lI, SDF-1J,
IP-10, Mig, GROI, GROJ, GROK, interleukin-8, PF4, ENA-78, GCP-2,
PBP, CTAP-III, J-thromboglobulin, NAP-2, C10, DC-CKI, CKIl, CKI2,
MCP-1, MCP-2, MCP-3, MCP-4, MIP-lI, MIP-1J, lymphotactin, ATAC,
eotaxin, eotaxin-2, I-309, HCC-1, HCC-2, HCC3, LARC/MIP-3I,
MIP-3J, PARC, TARC, 6Ckine, ELC, SLC, CKJ4, CKJ6, CKJ7, CKJ8,
CKJ9, CKJ11, CKJ12, CKJ13, and CX3C.
6. The composition of claim 1 wherein the polynucleotide is selected from
the group consisting of:
(a) a polynucleotide comprising the nucleotide sequence of SEQ ID
NO: 6;
(b) a polynucleotide comprising the nucleotide sequence of the
protein-coding sequence of the polynucleotide encoding met-
hDSF-1I deposited under accession number ATCC 98506;
(c) a polynucleotide encoding an amino-terminal-modified
chemokine comprising the amino acid sequence of SEQ ID NO:
10;
(d) a polynucleotide encoding a protein comprising an amino-
terminal fragment of the amino acid sequence of SEQ ID NO: 10;
(e) a polynucleotide comprising a nucleotide sequence
complementary to any one of the polynucleotides specified in (a)-
(d) above; and
(f) a polynucleotide capable of hybridizing at either (i) 4xSSC at
65°C or (ii) 50% formamide and 4XSSC at 42°C, to any one of
the polynucleotides specified in (a)-(e) above.
The examiner relies on the following reference:
Proudfoot et al. (Proudfoot), ”Extension of Recombinant Human RANTES by the
Retention of the Initiating Methionine Produces a Potent Antagonist,” J. Bio.
Chem., Vol. 271 No. 5, pp.2599-2603 (1996)
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