Interference 104,002
reading frames of one clone, MN390-2 (a.k.a. MO390-2 or mc13241) and found that
only one [reading frame] had no stop codons. Id. Drs. Gray and Godiska are said to
have “immediately recognized” that the continuous open reading frame of the MN390-2
clone encoded a chemokine based on (i) the similarity of the amino acid sequence with
those of other known chemokines; and (ii) the presence of a pair of cysteines near the
amino terminus of the protein. Id., p. 128. When the MN390-2 sequence was
compared with other sequences that had been reported in the public GenBank
database, the greatest sequence identity found was with a rat chemokine known as
MIP-1$. Id.; GB, p. 60, Material Fact 133; GR 75, para. 55.
With respect to the “practical utility” of the polynucleotide of the count, Godiska
argues that from January 30 to February 1, 1995, Dr. Godiska cloned and “PCR-
amplified” the cDNA fragment encoding the mature chemokine 390 (a.k.a. MDC).
GB, p. 134; GB, p. 72, Material Fact 156. Godiska further argues that from
February 3-8, 1995, Dr. Godiska gave the “PCR-amplified” 390 DNA to Ms. Linda
Watson, manager of the Histology laboratory at Icos Corporation, to make probes for
use in an in situ hybridization experiment. GB, p. 135; GB, p. 72, Material Fact 158.
Ms. Watson is said to have prepared antisense and sense probes to measure the
expression of the 390 gene in selected normal and diseased tissues.4 GB, p. 135;
GB, p. 73, Material Fact 159. Ms. Watson radiolabeled the probes and performed
4 The antisense probe is said to bind specifically to chemokine 390 RNA in tissue
samples; whereas, the sense probe is said to be a negative control. GB, p. 135;
GB, p. 73, Material Fact 159.
6
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 Next
Last modified: November 3, 2007