One advantage provided by the materials and methods defined herein is the
ability to create an immortalized and totipotent cell from virtually any type
of precursor cell. These precursor cells can be embryonic cells, cultured
embryonic cells, primordial germ cells, fetal cells, and cells isolated from the
tissues of adult animals, for example. Cells isolated from the kidney and ear
of an adult grown bovine have been utilized as precursor cells for the
generation of immortalized, totipotent cells.
Strelchenko, Application 09/357,445, Paper I (specification), p. 73,11. 22-28.
F 60. Strelchenko specifically describes treatments for reprogramming these non-totipotent cells:
An example ofmaterials and methods for converting non-totipotent cells into
totipotent cells is to incubate precursor cells with a receptor ligand cock-tail.
Receptor ligand cocktails are described hereafter.
Strelchenko Application 09/357,445, Paper I (specification), p. 12,11. 20-22,
F 61. Strelchenko refers to the materials and methods used to convert or reprogram non-totipotent
"precursor"cells to totipotent cells as "stirmilus:"
The term "stimulus" as used herein refers to materials and/or methods useful
for converting precursor cells into immortalized and/or totipotent cells. The
stimulus can be electrical, mechanical, temperature-related, and/or chemical,
for example. The stimulus may be a combination of one or more different
types of stimuli. As described herein in exemplary embodiments, placing
precursor cells in culture can be a sufficient stimulus to convert precursor
cells into immortalized and/or totipotent cells. A stimulus can be introduced
to precursor cells for any period of time that accomplishes the conversion of
precursor cells into immortalized and/or totipotent cells.
Strelchenko Application 09/357,445, Paper I (specification), p. 28,11, 6-14.
F 62. Strelchenko specifically notes that the non-embryonic precursor cells which may be
converted into totipotent cells includes differentiated cells such as somatic cells:
The term "non-embryonic cell" as used herein refers to a cell that is not
isolated from an embryo. Non-embryonic cells can be differentiated or
nondifferentiated. Non-embryonic cells can refer to nearly any somatic cell,
such as cells isolated from an ex utero, animal.
Strelchenko Application 09/357,445, Paper I (specification), p. 12,11. 27-30.
F 63. Strelchenko's specification also provides additional information relating to the formation of
totipotent cells from "precursor" cells:
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