Appeal No. 2003-1126 Page 2
Application No. 08/444,285
39. A nonhuman transgenic mammal whose somatic and germ cells
contain exogenous genetic material, wherein said material does not
include any virus-specific DNA and includes at least one
heterologous gene and a transcriptional control sequence operably
associated therewith, wherein said gene is expressed at a
detectable level in a plurality of said somatic cells or said germ
cells, where said genetic material is selected so that the normal
development of the embryo is not prevented by said material,
where said mammal is selected from the group consisting of
rodents, rabbits, goats, pigs, cattle, and sheep.
51. A nonhuman mammal characterized as having somatic and germ
cells that contain exogenous material that does not include any
virus-specific DNA, said material including at least one
heterologous gene and a transcriptional control sequence operably
associated therewith, said gene being expressible at a detectable
level in a plurality of said somatic cells or said germ cells of said
mammal under the control of said control sequence, said genetic
material being selected so that the normal development of the
embryo to term is not prevented by such material, said mammal
having been obtained by the following steps:
(a) introducing exogenous genetic material into a pronucleus
of a mammalian zygote by microinjection, said genetic material
including at least one heterologous gene and a control sequence
operably associated therewith, thereby obtaining a genetically
transformed zygote;
(b) transplanting an embryo derived from the genetically
transformed zygote into a pseudopregnant female; and
(c) allowing the embryo to develop to term;
or a mammal descended from a transgenic mammal so produced
which retains the heterologous gene in expressible form, where
said mammal is selected from the group consisting of rodents,
rabbits, goats, pigs, cattle, and sheep.
56. A method of producing a polypeptide or protein which comprises:
(a) producing a mammal capable of expressing said
polypeptide or protein at a detectable level by a method which
comprises
(i) introducing exogenous genetic material that does
not include any virus-specific DNA into a pronucleus of a
mammalian zygote by microinjection, said genetic material
including at least one heterologous gene and a
transcriptional control sequence operably associated
Page: Previous 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Next
Last modified: November 3, 2007