Appeal No. 2004-1770 Page 7
Application No. 09/895,050
As Appellant points out, although Hirschbein teaches the advantage of using
“dry” reagents during oligonucleotide synthesis, the reference uses the term “dry” to
mean that the solvents used do not contain water. Hirschbein does not suggest any
advantage to removing the (non-aqueous) solvent from the activator before adding the
monomer to be reacted. Hirschbein teaches, in fact, that the activator-containing
solution and the monomer-containing solution should be mixed. See column 12, lines
7-26:
The reaction is performed by adding a solution of the phosphoramidite
monomer and a solution of an activator (or a solution containing the
phosphoramidite monomer and the activator) to the reaction vessel. . . .
The monomer and the activator either can be premixed, mixed in the valve-
block of a suitable synthesizer, mixed in a pre-activation vessel and
preequilibrated if desired, or they can be added separately to the reaction
vessel.
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