Appeal 2006-0931
Application 10/312,054
1. In a method for removing a positively charged substance from an
aqueous liquid (I) containing said substance by contacting the liquid with a cation-
exchanger (1) under conditions permitting binding of said substance to said cation-
exchanger (1), optionally followed by a subsequent desorption of said substance,
the improvement comprising selecting said cation-exchanger to be capable of
(a) binding said substance by cation-exchange in an aqueous liquid reference
(II) at an ionic strength corresponding to 0.3 M NaC1 and
(b) permitting a break through [sic, breakthrough] capacity for said substance
≥ 200% of the breakthrough capacity of said substance for a reference
cation-exchanger (2) containing sulphopropyl groups –CH2CH2CH2SO2O⎯.
22. A cation-exchanger (1) comprising a plurality of cation-exchange
ligands attached to a support matrix said ligands containing a cation-exchange
group selected among sulphonate (-SO3⎯/-SO3H), sulphate (-OSO3⎯/-OSO3H),
carboxylate (-COO⎯/-COOH), phosphate (-OPO32⎯/-OPO3H⎯/-OPO3H2 )and
phosphonate (-PO32⎯/-PO3⎯H/-PO3H2), said cation-exchanger (1) having a
breakthrough capacity for at least one of the reference proteins selected from the
group consisting of human serum albumin, lysozym and IgG, which is ≥ 200% of
the corresponding breakthrough capacity obtained for a sulphopropyl cation-
exchanger (cation-exchanger 2) with essentially the same support matrix, degree of
substitution, counterion etc as cation-exchanger (1) and under essentially the same
running conditions as for determining the breakthrough capacity for cation-
exchanger (1);
wherein cation-exchangers in which each of the cation-exchange groups are bound
to a support matrix via a non-substituted straight chain of carbon atoms interrupted
at only one position by a thioether sulphur are excluded.
2
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