Appeal 2007-1623
Application 09/981,845
an acetylated peptide that is the same as SEQ ID NO: 11 except that it lacks
the C-terminal non-osteopontin amino acids.) The example shows that SEQ
ID NO: 11 and SEQ ID NO: 15 both promote the attachment and spreading
of osteoprogenitor cells, and that the activity of SEQ ID NO: 15 was
inhibited by antibodies to the αvβ3 receptor but was not inhibited by
antibodies to the CD44 or αβ1 receptors (id. at 54-55, Table 8).
DISCUSSION
1. CLAIMS
Claims 1-3, 5, and 6 are on appeal and read as follows:
1. An active ostopontin peptide fragment comprising an amino acid
sequence selected from the group consisting of SEQ ID NO:7, SEQ ID
NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ
ID NO:13, SEQ ID NO:14, and SEQ ID NO:15, wherein the peptide binds
to at least one integrin receptor on a cell surface selected from the group
consisting of ανβ3, ανβ5, 4β1, 2β1, VCAM, ICAM CD44, V3Vx.
2. The peptide fragment of claim 1, wherein the peptide increases cell
attachment to a biomaterial and increases cell spread.
3. The peptide fragment of claim 1, wherein the peptide binds to at least
one integrin receptor on a cell surface selected from the group consisting of
VCAM, ICAM CD44, and V3Vx.
5. The peptide fragment of claim 1 wherein the integrin(s) is selected
from the group consisting of ανβ3, ανβ5, 4β1, and 2β1.
6. The peptide fragment of claim 1 wherein the cell is selected from the
group consisting of osteoprogenitor cells, tumor cells, macrophages,
periosteal cells, endothelial cells, epithelial cells, eosinophils, stem cells,
limited potential precursor cells, precursor cells, committed precursor cells,
and differentiated cells.
3
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Last modified: September 9, 2013