Ex parte KISHORE et al. - Page 4




                    Appeal No. 93-2460                                                                                                                                    
                    Application No. 07/590,647                                                                                                                            


                              VI.  Claims 11 through 14, 22 through 24 and 30 through 32                                                                                  
                    stand rejected under 35 U.S.C. § 102(b) as anticipated by or, in                                                                                      
                    the alternative, under 35 U.S.C. § 103 as obvious over Comai or                                                                                       
                    Fillatti.                                                                                                                                             
                              We affirm Rejections I and IV, and reverse Rejections II,                                                                                   
                    III, V and VI.                                                                                                                                        


                                                            Background and Discussion                                                                                     
                              The present invention is directed to a DNA sequence which                                                                                   
                    encodes a novel 5-enolpyruvyl-3-phosphoshikimate (EPSP)                                                                                               
                    synthetase enzyme, a method of making the DNA sequence, a plant                                                                                       
                    cell transformed with said DNA sequence, a plant comprising said                                                                                      
                    DNA sequence, a seed produced by a transformed plant, a method                                                                                        
                    for producing glyphosate-tolerant plants, and a method for                                                                                            
                    controlling weeds in a field containing crops.  The novelty of                                                                                        
                    the present EPSP enzyme is primarily due to two codon changes in                                                                                      
                    a petunia-derived DNA sequence encoding said enzyme which result                                                                                      
                    in the production of an enzyme having an alanine residue                                                                                              
                    substituted for a glycine residue between amino acid positions 80                                                                                     
                    and 120 of the mature protein, and a threonine residue                                                                                                
                    substituted for the terminal alanine, which is located between                                                                                        
                    amino acid positions 170 and 210.  According to the                                                                                                   

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