Appeal No. 93-2460 Application No. 07/590,647 maize and rice, “is likely to be a problem for some years because, so far, the establishment of the appropriate cell cultures is an art that depends upon parameters beyond experimental control (Potrykus, page 540, section 12).” Answer, sentence bridging pp. 17-18. Having provided objective evidence to support her position, the burden now shifts to the appellants to demonstrate by way of rebuttal evidence that the application is enabling. In re Marzocchi, 439 F.2d at 223, 169 USPQ at 369. In their response, however, the appellants address the rejection only as it applies to claim 46. Brief, p. 21. The appellants contend that “suitable transformation methods for both dicot and monocot plants were known at the time of filing of the instant application” and they refer generally to a list of references provided in an amendment, filed December 11, 1991 (Paper No. 5). Brief, p. 21, lines 4-8. In addition, the appellants argue that the teachings on pp. 66-68 of the specification demonstrate that techniques known in the art were used for the regeneration of maize protoplasts which expressed the claimed variant EPSPS. Thus, the appellants urge that it would not require undue experimentation for one skilled in the art to make and use the invention described in claim 46. We find the appellants’ position untenable. 1515Page: Previous 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 NextLast modified: November 3, 2007