Ex parte KISHORE et al. - Page 15




                    Appeal No. 93-2460                                                                                                                                    
                    Application No. 07/590,647                                                                                                                            


                    maize and rice, “is likely to be a problem for some years                                                                                             
                    because, so far, the establishment of the appropriate cell                                                                                            
                    cultures is an art that depends upon parameters beyond                                                                                                
                    experimental control (Potrykus, page 540, section 12).”  Answer,                                                                                      
                    sentence bridging pp. 17-18. Having provided objective evidence                                                                                       
                    to support her position, the burden now shifts to the appellants                                                                                      
                    to demonstrate by way of rebuttal evidence that the application                                                                                       
                    is enabling.  In re Marzocchi, 439 F.2d at 223, 169 USPQ at 369.                                                                                      
                              In their response, however, the appellants address the                                                                                      
                    rejection only as it applies to claim 46.  Brief, p. 21.  The                                                                                         
                    appellants contend that “suitable transformation methods for both                                                                                     
                    dicot and monocot plants were known at the time of filing of the                                                                                      
                    instant application” and they refer generally to a list of                                                                                            
                    references provided in an amendment, filed December 11, 1991                                                                                          
                    (Paper No. 5).  Brief, p. 21, lines 4-8.  In addition, the                                                                                            
                    appellants argue that the teachings on pp. 66-68 of the                                                                                               
                    specification demonstrate that techniques known in the art were                                                                                       
                    used for the regeneration of maize protoplasts which expressed                                                                                        
                    the claimed variant EPSPS.  Thus, the appellants urge that it                                                                                         
                    would not require undue experimentation for one skilled in the                                                                                        
                    art to make and use the invention described in claim 46.  We find                                                                                     
                    the appellants’ position untenable.                                                                                                                   

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