Appeal No. 93-2460
Application No. 07/590,647
where X is either alanine, serine or threonine, where said
1
second amino acid sequence is located between positions 170 and
210 in the EPSP synthase gene.
5. The gene of Claim 4 encoding a protein as shown in
Figure 1 but which protein contains the first and second amino
acid sequences.
6. A gene encoding a glyphosate-tolerant EPSP synthase
produced by the method of Claim 1 wherein the EPSP synthase gene
in which the codon substitutions are made is selected from the
group of EPSP synthase consisting of petunia, tomato, maize,
Arabidopsis thaliana, soybean, Brassica napus, E. coli K-12, and
Salmonella typhimurium proteins as shown in Figure 1.
7. A plant gene encoding a glyphosate-tolerant EPSP
synthase enzyme of Claim 4.
8. A plant transformation vector comprising a gene which
encodes a glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate
(EPSP) synthase having a first amino acid sequence:
-L-G-N-A-A-T-A-
located between positions 80 and 120 of the mature EPSP synthase
sequence and a second amino acid sequence
-A-L-L-M-X -P-L-T-
1
wherein X is either alanine, serine or threonine, where said
1
second amino acid sequence is located between positions 170 and
210 in the mature EPSP synthase sequence.
9. A vector of Claim 8 containing a glyphosate-tolerant
plant EPSP synthase.
10. A vector of Claim 8 containing a glyphosate-tolerant
bacterial EPSP synthase.
11. A transformed plant cell containing a gene of Claim 7.
22
Page: Previous 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Next
Last modified: November 3, 2007