Appeal No. 95-0285
Application No. 07/648,586
Paper No. 33, filed March 3, 1998. Accordingly, the appeal with
respect to claim 11 is dismissed, leaving claims 1 through 10, 12
through 14, 16 and 18 through 23 for our consideration.
Claims 1, 10, 12 and 19 are representative:
1. An assay kit for identifying periodontal disease in a
patient, said disease correlated to elevated levels of aspartate
aminotransferase (AST) in a crevicular fluid sample from the
patient, which kit comprises an enclosure containing:
an aliquot of a buffered aqueous solution of cysteine
sulfinic acid (CSA) provided in a container for said aliquot;
a plurality of solid indicator supports each comprising a
triarylmethine dye affixed to a solid matrix, said dye reactive
with sulfite ion and nonreactive with both CSA and AST; and
an assay plate provided with a plurality of wells, each well
defining a volume sufficient to hold one of said solid indicator
supports and a portion of the CSA solution adequate to perform at
least one assay. [Emphasis added.]
10. An assay kit for identifying a disease correlated to
elevated levels of aspartate aminotransferase (AST) in a bodily
fluid sample from a patient, which kit comprises an enclosure
containing:
an aliquot of a buffered aqueous solution of cysteine
sulfinic acid (CSA) provided in a container for said aliquot; and
an assay plate defining a plurality of assay wells, said
assay wells containing a triarylmethine dye that is reactive with
sulfite ion but nonreactive with CSA and AST, each of said assay
wells defining a volume sufficient to hold the fluid sample and a
portion of the CSA solution adequate to perform at least one
assay. [Emphasis added.]
12. A method for determining the amount of aspartate
aminotransferase (AST) in a body fluid sample from a mammal,
which method comprises:
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