Walker Table 2 Data - La v. B* Ab*
500
450
400
350
300
a
250
L
200
150
100
50
0
0 1000 2000 3000 4000 5000 6000
B*
Graph 3
The arrow in the upper right hand corner of each graph identifies Walker’s Ab* data points,
the points corresponding to a great excess of ligand. In Graphs 2 and 3, for the first nine and six data
*
points, respectively, the increase in B is proportional to the increase in concentration L of the ligand,
a
i.e., a relatively small increases in the concentration of antigen (L ) results in a relatively large
a
increases in the assay value B*. But for the Ab* data points shown in Graphs 2 and 3, a very large
increase in concentration, L , results in a disproportionately small increase in the B* value. Thus, in
a
Graph 3, a little over a 3-fold increase in concentration from 7.5 to 25 µg/liter resulted in a little over
2-fold increase in B*, from 1650 to 3700 counts per minute. The nearly 7-fold increase from 75 to
500 µg/liter, the Ab* concentration, resulted an increase of only ten percent (5000 to 5500
counts/minute).
31
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Last modified: November 3, 2007