For comparison we have also plotted Engvall’s example 1 data on Graph 4 below.
Engvall Example 1 Data - La v. B* Ab*
100
y = 63.156x - 1.3475
90
R2 = 0.999
80
70
60
a
L 50
40
30
20
10
0
0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6
B*
Graph 4
The arrow at the upper right Graph 4 indicates the Ab* value of 1.6 used by Bergland, the
highest value reported in Engvall’s example 1. E128, p. 3-4. As can be seen from the plot, the Ab*
value, 1.6, is proportional with the other reported values. In other words, the value of 1.6 is not
near saturation and does not appear to be a assay value which represents a sample having a great
excess of ligand. Engvall’s value of 1.6 does not meet Walker’s criteria of a limiting Ab* value.
E57, p.588. Thus, the person having ordinary skill in the art would not know to a reasonable degree
of certainty, what value to use for Ab*. The value for the affinity constant determined by Bergland
from a Scatchard analysis of Engvall’s example 1 data is speculative.
Thus, there is little certainty that the person having ordinary skill in the art would similarly
9
arrive at a value of 3.7 x 10 liters/mole applying a Scatchard plot analysis to the data in Engvall’s
example 1.
32
Page: Previous 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 Next
Last modified: November 3, 2007