the lower limit set forth in the claim. The recalculated data and the Scatchard Plot are shown in the
Table and in Graph 1 below:
Scatchard Plot Data from Example 1 using Background Absorbance of .02
L (µg/liter) Absorbance B* B*/Ab* Ab*/B* w =1-Ab*/B* L ·w
a 2 a 2
3.1 0.07 0.05 0.03125 32 -31 -96.100
6.25 0.13 0.11 0.06875 14.5455 -13.5455 -84.659
12.5 0.25 0.23 0.14375 6.9565 -5.9565 -74.457
25 0.44 0.42 0.2625 3.8095 -2.8095 -70.238
50 0.82 0.8 0.5 2 -1 -50.000
100 1.6
Scatchard Plot using Background Absorbance of .02
La·w2
-100.000-90.000 -80.000 -70.000-60.000 -50.000 -40.000-30.000 -20.000-10.000 0.000
1.000
0.900
0.800
0.700
0.600
0.500
0.500B*/Ab*
0.400
0.300
0.263
0.200
0.144
0.100
0.031 0.069
0.000
Graph 1
28
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Last modified: November 3, 2007