Interference No. 102,572
40).
(14) Perry indicated that the first sample she analyzed from a "cotransformed
refractile body preparation" was supplied to her by Cabilly. She analyzed this sample by
PAGE. Perry, ¶ 12 (CR-26).
(15) Cabilly testified that he also constructed pGammaCEAFABtrp207-1*, a
plasmid vector for the direct expression of the FAB fragment of the heavy chain gene.
(CR-40). Accordingly to Cabilly, he digested pBR322 with HindIII, filled in, digested with
PstI and treated with BAP. He isolated the vector fragment by PAGE (fragment I). Cabilly,
¶ 9. Cabilly indicates that he received a sample of pGammaCEAtrp207-1* from Holmes,
he digested this plasmid with BamHI and PstI and isolated the fragment by PAGE
(fragment II). Another sample of this plasmid was digested with NcoI and NdeI, he isolated
the 260 bp DNA by PAGE. He used a 13 bp oligonucleotide primer in a primer repair
reaction in order to introduce a termination codon. The fragment was then digested with
BamHI, the 179 bp fragment isolated by PAGE, filled in (fragment III). Fragments I, II and III
were ligated and transformed into E. coli. Cabilly, ¶ 9. These transformants were said to
be analyzed by Rey, Holmes and Cabilly by restriction cleavage analysis and
15
sequencing. Cabilly, ¶ 9, Holmes, ¶ 20 and Rey, ¶ 7 (CR-40-41).
id.
15The Cabilly et al. brief (page 14) alleges that this analysis was performed on or
about January 22, 1983. No one testified as to this date for the analysis. id.
17
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