Interference No. 102,572
8
with Shively who was involved in the study of human anti-CEA antibodies and that he
requested and received from Shively, on
or about February, 1981, a mouse hybridoma cell line, CEA.66-E3, said to express anti-
CEA antibodies. Riggs, ¶¶ 3-5 (CR-15-16).
(2) Shively testified that he had conversations with Riggs regarding the project and
that upon request from Riggs he supplied Riggs with the cells requested on or about
February, 1981. Shively, ¶ 5 (CR-17).
(3) Cabilly, a post doctoral fellow in Riggs' laboratory at City of Hope, testified that
in September 1981, he received CEA.66-E3 cells from Shively and that he used these
cells to extract total RNA. The polyA mRNA was purified from the total RNA by using an
oligo-dT cellulose column. Following the isolation of the mRNA, Cabilly testified that he
gave a sample of it to Holmes at Genentech for the preparation of an E. coli colony cDNA
library. Cabilly ¶ 4 (CR-39).
(4) Coinventor Holmes testified that on July 12, 1982 he began working on the
project to express antibodies directed against human CEA in E. coli. Holmes, ¶ 3.
Holmes testified that he received a sample of polyA mRNA from City of Hope and
prepared cDNA which was incorporated into plasmids to make a E. coli colony library.
Holmes, ¶ 4. He testified that he inoculated colonies into microliter plates and the cultures
8Carcinoembryonic antigen (CEA) is an antigen unique to humans and is found
mainly in intestinal tumors. (CX-2, page 2).
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