Appeal No. 1995-2518
Application 07/895,581
An understanding of the invention can be derived from a reading of claims 51, 52,
and 53, which are reproduced below:
51. A Saccharomyces cerevisiae strain which lacks carboxypeptidase ysc"
activity and lacks proteolytic activity selected from the group consisting of proteolytic yscA,
yscB, yscY and yscS activity and has been transformed with a hybrid vector comprising a
Saccharomyces cerevisiae promoter operably linked to a DNA coding for a full-length
mature protein which bears no basic C-terminal amino acids and which is selected from
the group consisting a hANP, EGF, connective tissue activating peptide-III and
desulphatohirudin.
52. A method for the production of a Saccharomyces cerevisiae strain which lacks
carboxypeptidase ysc" activity and lacks proteolytic activity selected from the group
consisting of proteolytic yscA, yscB, yscY and yscS activity and has been transformed with
a hybrid vector comprising a Saccharomyces cerevisiae promoter operaly linked to a DNA
coding for a full-length mature protein which bears no basic C-terminal amino acids and
which is selected from the group consisting of hANP, EGF, connective tissue activating
peptide-III and desulphatohirudin, comprising transforming a saccharomyces cerevisiae
strain which lacks carboxypeptidases ysc" activity and lacks proteolytic activity selected
from the group consisting of proteolytic yscA, yscB, yscY and yscS activity with said hybrid
vector.
53. A method for the production of a full-length mature protein which bears no basic
C-terminal amino acids and which is selected from the group consisting of hANP, EGF,
connective tissue activating peptide-III and desulphatohirudin, comprising culturing a
Saccharomyces cerevisiae strain which lacks carboxypeptidase ysc" activity and lacks
proteolytic activity selected from the group consisting of proteolytic yscA, yscB, yscY and
yscS activity and has been transformed with a hybrid vector comprising a Saccharomyces
cerevisiae promoter operably linked to a DNA coding for said protein, and isolating said
protein.
The references relied upon by the examiner are:
Bussey et al. (Bussey) 4,929,553 May 29, 1990
Hinnen et al. (Hinnen) EPA 0,213,593 Mar. 11, 1987
Meyhack et al. (Meyhack) EPA 0,225,633 Jun. 16, 1987
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