Appeal No. 1995-2518 Application 07/895,581 An understanding of the invention can be derived from a reading of claims 51, 52, and 53, which are reproduced below: 51. A Saccharomyces cerevisiae strain which lacks carboxypeptidase ysc" activity and lacks proteolytic activity selected from the group consisting of proteolytic yscA, yscB, yscY and yscS activity and has been transformed with a hybrid vector comprising a Saccharomyces cerevisiae promoter operably linked to a DNA coding for a full-length mature protein which bears no basic C-terminal amino acids and which is selected from the group consisting a hANP, EGF, connective tissue activating peptide-III and desulphatohirudin. 52. A method for the production of a Saccharomyces cerevisiae strain which lacks carboxypeptidase ysc" activity and lacks proteolytic activity selected from the group consisting of proteolytic yscA, yscB, yscY and yscS activity and has been transformed with a hybrid vector comprising a Saccharomyces cerevisiae promoter operaly linked to a DNA coding for a full-length mature protein which bears no basic C-terminal amino acids and which is selected from the group consisting of hANP, EGF, connective tissue activating peptide-III and desulphatohirudin, comprising transforming a saccharomyces cerevisiae strain which lacks carboxypeptidases ysc" activity and lacks proteolytic activity selected from the group consisting of proteolytic yscA, yscB, yscY and yscS activity with said hybrid vector. 53. A method for the production of a full-length mature protein which bears no basic C-terminal amino acids and which is selected from the group consisting of hANP, EGF, connective tissue activating peptide-III and desulphatohirudin, comprising culturing a Saccharomyces cerevisiae strain which lacks carboxypeptidase ysc" activity and lacks proteolytic activity selected from the group consisting of proteolytic yscA, yscB, yscY and yscS activity and has been transformed with a hybrid vector comprising a Saccharomyces cerevisiae promoter operably linked to a DNA coding for said protein, and isolating said protein. The references relied upon by the examiner are: Bussey et al. (Bussey) 4,929,553 May 29, 1990 Hinnen et al. (Hinnen) EPA 0,213,593 Mar. 11, 1987 Meyhack et al. (Meyhack) EPA 0,225,633 Jun. 16, 1987 2Page: Previous 1 2 3 4 5 6 7 8 9 10 NextLast modified: November 3, 2007