Appeal No. 1995-2518
Application 07/895,581
heterologous protein. The preferred yeast strain is also described as being defective in at
least one protease activity selected from yscA, yscB, yscY and yscS which serves to
further reduce random proteolysis of the protein product. The invention is said to include
the process of producing the protein, the genetically engineered yeast cells and the
methods of preparing the yeast cells.
Discussion:
Claims:
Claim 51 is directed to a Saccharomyces cerevisiae strain which lacks
carboxypeptidase ysc" acid and lacks proteolytic activity selected from the group
consisting of proteolytic yscA, yscB, yscY and yscS. The strain has been transformed with
a hybrid vector comprising a S. cerevisiae promotor operably linked to a DNA which will
encode a full-length mature protein having no basic C-terminal amino acids wherein the
protein is selected from the group consisting of hANP, EGF, connective tissue activating
peptide-III and desulphatorhirudin. Claim 52 is directed to a method of producing a S.
cerevisiae strain claimed in claim 1 and claim 53 is directed to a method of producing a
full-length mature protein using a S. cerevisiae strain claimed in claim 1.
The rejections under 35 U.S.C. § 103
Claims 51-55 and 57-61 stand rejected under 35 U.S.C. § 103 as being
unpatentable over Bussey taken with Achstetter, Hinnen and Meyhack.
Claim 56 stands rejected under 35 U.S.C. § 103 as being unpatentable over
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