Appeal No. 95-4473
Application 08/003,894
Claims 1 and 9, which are illustrative of the subject matter on appeal, read as
follows:
1. A method for determining the presence of bone alkaline phosphatase in a
sample suspected of containing said bone alkaline phosphatase, which comprises:
(a) bringing together in an aqueous medium:
(1) said sample;
(2) a first antibody capable of specifically binding to a first
epitopic site on said bone alkaline phosphatase, and
(3) a second antibody capable of specifically binding to a
second epitopic site on said bone alkaline phosphatase,
wherein said first and second epitopic sites are different and said
first and second antibodies together form an immunocomplex with said bone
alkaline phosphatase, if present, and wherein said first and second
antibodies exhibit less than 1% cross-reactivity with liver alkaline
phosphatase and are selected from the group consisting of a monoclonal
antibody capable of binding to the epitopic site recognized by a monoclonal
antibody produced by hybridoma cell line ATCC No. HB 11106, a
monoclonal antibody capable of binding to the epitopic site recognized by a
monoclonal antibody produced by hybridoma cell line ATCC No. HB 11107,
and a monoclonal antibody capable of binding to the epitopic site
recognized by a monoclonal antibody produced by hybridoma cell line ATCC
No. HB 11108, with the proviso that said first and second antibodies are
different; and
(b) examining said medium for the presence of said immunocomplex, the
presence thereof being related to the presence of bone alkaline
phosphatase in said medium.
9. A method for determining the presence of bone alkaline phosphatase in a
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Last modified: November 3, 2007