Appeal No. 95-4473
Application 08/003,894
sample suspected of containing said bone alkaline phosphatase which comprises;
(a) bringing together in an aqueous medium:
(1) said sample;
(2) a first antibody capable of specifically binding to a first
epitopic site on said bone alkaline phosphatase,
(3) a second antibody capable of specifically binding to a
second epitopic site on said bone alkaline phosphatase,
wherein said first and second epitopic sites are different and
said first and second antibodies together form an immunocomplex
with said bone alkaline phosphatase, if present, and wherein said first
and second antibodies are selected from the group consisting of a
monoclonal antibody produced by hybridoma cell line ATCC No. HB
11106, a monoclonal antibody produced by hybridoma cell line ATCC
No. HB 11107 and a monoclonal antibody produced by hybridoma
cell line ATCC No. HB 11108, with the proviso that said first and
second antibodies are from different cell lines; and
(b) examining said medium for the presence of said immunocomplex, the
presence thereof being related to the presence of bone alkaline
phosphatase in said medium.
The references relied on by the examiner are:
Hill et al. (Hill) EP 0 381 450 Aug. 08, 1990
Nagoya et al., "Detection of Bone-type Alkaline Phosphatase by Monoclonal
Antibodies Reacting With Human Osteosarcoma-associated Antigen", Jpn.
Cancer Res., Vol. 82, pp. 862-870, July 1991. (Nagoya)
Previously entered rejections under 35 USC § 112, first and second paragraphs,
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