Appeal No. 1995-4717
Application 07/876,288
We reverse.
BACKGROUND
The invention is described at pages 3-4 of the specification as being directed to a
method of generating a random peptide library wherein host cells are transformed with a
set of recombinant DNA vectors that code for the expression of a tripartite fusion protein
made up of a carrier protein, a peptide, and a proteolytic cleavage site between the carrier
protein and the peptide. The transformed host cells are then cultured under conditions
suitable for expression of the fusion protein. The peptide library is said to be useful in the
identification of peptides that bind to receptor molecules of interest.
DISCUSSION
The Claims:
Claim 1 is directed to a two step method of generating a random peptide library.
The initial step comprises transforming host cells with a set of recombinant DNA vectors.
Each vector encodes a different tripartite fusion protein which consists essentially of a
carrier protein, a peptide and a proteolytic cleavage site. The vectors differ from one
another with respect to the peptide encoded. The peptide portion of the fusion protein can
be "specified exactly, completely random, composed of the production of building variation
around a core or lead sequence, either immobilized or a free peptide, or presented in a
3
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