Appeal No. 95-5069
Application No. 08/202,055
REPRESENTATIVE CLAIM
Claim 1, which is illustrative of the subject matter on
appeal, reads as follows:
1. A method for modifying the carbohydrate moiety on
glycoproteins comprising:
(a) treating glycoprotein-secreting CHO cells having
low endomannosidase activity under cell
maintenance conditions with N-butyl-
deoxynojirimycin under non-denaturing
conditions;
(b) secreting and purifying the glycoprotein from
the cells of step (a); and
(c) treating the secreted and purified glycoprotein
from step (b) with endoglycosidase H under non-
denaturing conditions to provide a glycoprotein
with a single GlcNAc residue at each
glycosylation sequon, thereby facilitating the
structural and functional analysis of said
secreted and purified glycoprotein.
THE REFERENCES
In rejecting the appealed claims on non-prior art
grounds, the examiner relies on these references:
Nadine Peyrieras et al. (Peyrieras), "Effects of the
glucosidase inhibitors nojirimycin and deoxynojirimycin on the
biosynthesis of membrane and secretory glycoproteins," 2 The
EMBO Journal
no. 6, 823-32 (1983)
Pedro A. Romero and Annette Herscovics (Romero), "Transfer of
Nonglucosylated Oligosaccharide from Lipid to Protein in a
-3-
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