Interference No. 103,203
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-- prior to the computer printout that I received on February 3, ‘87, I believed that the
protein terminated at Histidine 259. The computer printout of that date clearly
shows an open reading frame going out to 263 followed by a stop codon. And I
have indicated on that computer printout a question mark, circling a cytosine
residue, and written -- above that I have written a “typo ? ?,” meaning was this the
correct sequence or was this a typographical error on input of the data into the
computer.
My recollection is that on the previous week we had written the sequence out
to Histidine 259 and -- followed by the stop codon. I believed at that point, which
was up -- the week preceding the weekend of the end of January, beginning of
February, that we had finished the sequence and had the correct sequence as of --
out to 259.
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On Tuesday, which is February 3rd, I received a new computer printout, which
indicated a different carboxy terminal in the protein. That surprised me, that is why I
made the marking on the computer printout questioning whether that residue, in
fact, was a cytosine or a typographical error. On rechecking of the data that was
inputted into the computer, it clearly was not a typographical error, therefore, I
changed this document before me, [MS&Y] 7693, to reflect the fact that the protein -
- that the DNA sequence indicated that the protein sequence terminated at 263
rather than 259 [emphasis added] [NR 110, line 3- 111, line 18].
Dr. Bach testifies that he informed Drs. Nemerson and Konigsberg as well as other
members of the group, in particular, Drs. Spicer, Bloem and Horton, on February 3 and 4,
1987, that the tissue factor protein was 263 amino acids in length, and not 259. NR 112,
line 8- NR 113, line 1.
Conspicuous in its absence, is any explanation by Dr. Bach as to how he was able
to determine the complete and correct nucleotide sequence of amino acids 1 to 263 of
human tissue factor from the computer printout of the nucleotide sequence which was said
to be in his possession; i.e., from NRE 278. That is, Dr. Bach has not explained how he
was able to generate a complete and correct handwritten sequence (both nucleotide and
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