Interference No. 103,203
two errors in the nucleotide sequence shown in the computer printout of February 14,
1987, NRE 282, because the correct amino acid sequence shifts from reading frame A at
approximately nucleotide 347 (amino acid 47 of mature tissue factor) to reading frame B
and shifts back to being the correct amino acid in reading frame A at approximately amino
acid 82 (nucleotide 450). Dr. Horton acknowledges that because of the errors in the
sequence, the amino acid sequence of the tissue factor protein is not the same as
Nemerson’s final published version shown in NRE 64. NR 1961-1970. Dr. Horton
eventually concedes that the nucleotide sequence shown in NRE 282 is not the correct
sequence coding for human tissue factor. NR 1970. Thus, we find Dr. Horton’s testimony
to be inconsistent with Dr. Bach’s, and fails to corroborate Dr. Spicer’s, testimony that
Nemerson et al. were in possession of the nucleotide sequence encoding the complete
tissue factor protein on February 3, 1987. To the contrary, the computer printout, NRE
278, indicates that as late as February 14, 1987, Nemerson et al. still were not in
possession of a species within the scope of count 2.
As to the testimony of Dr. Konigsberg, we find that he states that during a laboratory
meeting on February 4, 1987, Dr. Bach showed Nemerson Exhibit 277. NR 1982. Dr.
Konigsberg further states that as a result of the discussions during said meeting they
decided to (i) rerun some of the sequencing gels to confirm an ambiguity between the
DNA and protein sequence, and (ii) rerun some other sequencing gels
of the subclones where the DNA was in a reverse orientation to determine whether
the frameshift interpretation was due to G compression, thus leading to an apparent
error in the DNA sequence. From the results of this experiment, we also hoped to
confirm that the carboxy-terminus of human tissue factor beginning at amino acid
residue 258, consisted of Ser-Pro-Leu-Asn-Val-Ser, rather than His-Ser [NR 1983].
47
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