Appeal No. 1996-0848
Application 07/624,053
22. A continuous cell line producing a human monocyte cytotoxicity inducing
factor, the factor characterized by the following properties:
(a) capability of inducing human monocytes to a cytotoxic state;
(b) retention of biological activity following treatment at pH 2 for one hour;
B
(c) retention of biological activity following treatment at 60 C for one hour;
(d) ability to bind to Matrex Gel Red A under low-salt conditions and elute
from Matrex Gel Red A under high-salt conditions; and
(e) retention of biological activity in the presence of antiserum to interferon
gamma, interferon alpha, or a combination of anti-sera to interferon alpha
and gamma.
25. A continuous cell line which produces a factor capable of inducing human
monocytes to a cytotoxic state, the continuous cell line being produced by a process
comprising the steps of:
(a) immortalizing human T-cells to produce continuous cell clone;
(b) identifying a clone which produces a human monocyte cytotoxicity inducing factor
having the following properties:
i) capability of inducing human monocytes to a cytotoxic state;
ii) retention of biological activity following treatment at pH 2 for one hour;
B
iii) retention of biological activity following treatment at 60 C for one hour;
iv) ability to bind to Matrex Gel Red A under low-salt conditions and elute
from Matrix Gel Red A under high-salt conditions; and
2
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