Appeal No. 1996-2442
Application 08/062,021
said first and second capture probes having Tm’s which differ by no more
than about 15°C, and
C) simultaneously detecting said captured amplified hCMV DNA strand and
said captured amplified second target DNA strands as a simultaneous determination of
the presence of hCMV DNA and said second target DNA.
37. A diagnostic element comprising a water-insoluble, heat or ultrasonic
sealable support, having disposed thereon in distinct regions thereof, a plurality of
capture reagents,
each capture reagent having a capture probe specific for and hybridizable
with a distinct target DNA at a temperature from about 40 to about 55°C, each of said
capture probes having from 10 to 40 nucleotides and a Tm greater than about 50°C,
and the Tm’s of all capture probes differing by no more than about 15°C,
at least one of said capture probes being specific for and hybridizable with
hCMV DNA.
38. An oligonucleotide having a sequence selected from the group consisting
of:
SEQ ID NO: 3 TGCACTGCCA GGTGCTTCGG CTCAT,
SEQ ID NO: 16 CATTCCCACT GACTTTCTGA CGCACGT,
SEQ ID NO: 17 TGAGGTCGTG GAACTTGATG GCGT,
SEQ ID NO: 18 GGTCATCGCC GTAGTAGATG CGTAAGGCCT,
SEQ ID NO: 19 GGAATGACGC AAGGACATAT GGGCGT,
SEQ ID NO: 20 CCCAGGTGCA CACCAATGTG GTGGAT,
SEQ ID NO: 21 GGACTGTGCG CGTTGTATAC CCTGC,
SEQ ID NO: 22 ACTCCCGAAG CGAATGGCAC GTGGA,
SEQ ID NO: 23 CATAGCTTGT GCCCGTGTGG CACGT,
SEQ ID NO: 24 CCAAGACGAG ACCGTCAGAG CTGGT,
SEQ ID NO: 25 AAGCTGTTGC CGCCATCAAA TAAACG, and
SEQ ID NO: 26 CTGCGTTAGA CCGAGAACTG TGGATAAAGG
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