Appeal No. 1997-0160
Application No. 08/073,985
13. A method for genus-specific and species-specific detection of
bacteria in a sample liquid, comprising the steps of:
hybridizing RNA of target bacteria with a primer which is
complementary to a genus-specific region of RNA of said target bacteria,
wherein the 3' end of said primer is only complementary to the RNA of said
target bacteria and not complementary to the RNA of other bacteria in said
sample which differ from the genus of said target bacteria;
elongating said primer in the presence of a suitable polymerase and
the four deoxyribonucleotides to form an elongation product;
labeling said elongation product concurrently with the elongation of
said elongation product or subsequent to said elongation;
hybridizing said elongation product with a species-specific labeled
oligonucleotide after denaturation to form a hybridization elongation product;
and
detecting said hybridization elongation product.
The references relied on by the examiner are:
Mullis et al. (Mullis) 4,683,195 Jul. 28, 1987
WIPO Int. App. (Hogan) 88/03957 Jun. 2, 1988
Frohman et al. (Frohman), "Rapid production of full-length cDNAs from rare transcripts:
Amplification using a single gene-specific oligonucleotide primer," Proceedings of the
National Academy of Sciences, USA, Vol. 85, pp. 8998-9002 (December 1988).
Appellants rely on the following references in their brief (pages 13 and 18):
3
WIPO Int. App. (Hogan and Kohne) 84/02721 Jul. 19, 1984
3Hogan and Kohne was made of record in the Information Disclosure Statement filed July 12, 1995
(Paper No. 38) by appellants.
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