Appeal No. 1997-2139
Application No. 08/114,595
Claims 1, 7, 23, 25-27, 30 and 49 are illustrative and read as follows.
1. An adenovirus or herpes virus vector construct comprising a
recombinant insert including an expression region comprising an essential
adeno-associated virus (AAV) gene, the vector expressing an essential
AAV protein.2
7. The vector construct of claim 1, wherein the vector is a herpes
simplex virus (HSV), a cytomegalovirus (CMV), a pseudorabies virus
(PRV) or an Epstein-Barr Virus (EBV) vector .
23. A recombinant host cell incorporating a vector construct in
accordance with claim 1.
25. The recombinant host cell of claim 23, wherein the cell further
includes a recombinant AAV vector integrated into its genome.
26. The recombinant host cell of claim 25, wherein the AAV vector
includes AAV ITR [i.e., inverted terminal repeat] sequences and an
expression region encoding an exogenous protein.
27. The recombinant host cell of claim 26, wherein the AAV vector
includes an expression region encoding a full length cystic fibrosis
transmembrane conductance regulator (CFTR) protein.
30. A method of producing recombinant AAV virions, comprising
the steps of:
(a) preparing a recombinant adenovirus or herpes virus which
includes a vector construct comprising an essential AAV
2 "The terms 'essential AAV genes' and 'essential AAV protein' are intended to refer to those
genes, and their encoded proteins, which are normally encoded by wild type AAV and are required for
AAV replication, genome packaging and virion assembly. Naturally, when intended for use in AAV
production, the adenoviral or herpes virus vector will be constructed so the inserted AAV genes
complement any essential AAV genes which have been deleted from a recombinant AAV vector to allow
an exogenous gene, such as a therapeutically important gene, to be inserted into the AAV vector."
[Specification, para. bridging pp. 7-8.]
"The ITR sequences are the only essential cis-acting elements for an AAV vector to mediate
genome packaging and integration into host cells" (specification, p. 6, ll. 31-33), while "the AAV genes
(rep-lip-cap) [are] essential for replication" (specification, p. 21, l. 11).
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