Appeal No. 1997-2139
Application No. 08/114,595
gene expression region, the vector expressing an essential
AAV protein;
(b) preparing a cell capable of producing AAV by
introducing a recombinant AAV vector into a host cell;
(c) infecting said cell with said recombinant virus in an amount
effective to stimulate the production of recombinant AAV
virions, and
(d) culturing said infected cell to obtain the recombinant AAV
virions.3
49. A recombinant AAV virion prepared by the process of claim 30,
which includes a vector construct comprising a nucleic acid sequence
encoding a full length CFTR protein, the vector capable of expressing the
entire CFTR protein.
The references relied on by the examiner are:
Post et al. (Post), "A Generalized Technique for Deletion of Specific Genes in Large
Genome: " Gene 22 of Herpes simplex Virus 1 Is Not Essential for Growth," Cell,
Vol. 25, pp. 227-32 (1981)
Haj-Ahmad et al. (Haj-Ahmad), "Development of a Helper-Independent Human
Adenovirus Vector and Its Use in the Transfer of the Herpes Simplex Virus Thymidine
Kinase Gene," Journal of Virology, Vol. 57, No. 1, pp. 267-74 (1986)
Drumm et al. (Drumm), "Correction of the Cystic Fibrosis Defect In Vitro by Retrovirus-
Mediated Gene Transfer," Cell, Vol. 62, pp. 1227-233 (1990)
Muzyczka, "Use of Adeno-Associated Virus as a General Transduction Vector for
Mammalian Cells," Current Topics in Microbiology and Immunology, Vol. 158, pp.
97-129 (1992)
3 A virion is the complete viral particle, which may or may not have an envelope, which serves to
transfer the viral nucleic acid from one cell to another (see MOLECULAR BIOLOGY AND TECHNOLOGY:
A Comprehensive Desk Reference, p. 953 (Robert A. Meyers, ed.,VCH Publishers, Inc., New York, 1995)
(copy attached).
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