Appeal No. 1997-2532
Application No. 08/427,569
23. A set of synthetic oligonucleotides useful as amplifier probes in a
sandwich hybridization assay for HTLV-1, comprising at least two different
oligonucleotide probes, wherein each oligonucleotide probe consists of:
a first segment having a minimum length of about 25 nucleotides and
a maximum length of about 100 nucleotides which segment is at least 90%
homologous to a segment of HTLV-1 nucleic acid, wherein said first
segment comprises a nucleotide sequence selected from the group
consisting of SEQ ID NOS: 6-41; and
a second segment consisting of a nucleotide sequence which is at
least 90% homologous to an oligonucleotide segment of a nucleic acid
multimer wherein said second segment is not complementary to HTLV-1
nucleic acid;
and optionally one or more noncomplementary segments each
consisting of a nucleotide sequence that is not complementary to HTLV-1
nucleic acid.
31. A solution sandwich hybridization assay for detecting the
presence of HTLV-1 in a sample, comprising:
(a) contacting the sample with (i) amplifier probes comprising the set
of synthetic oligonucleotides of claim 23 and (ii) a set of capture probe
oligonucleotides wherein there is a molar excess of amplifier probes and of
capture probes over analyte nucleic acid in the sample, wherein said set of
capture probe oligonucleotides comprises at least two different
oligonucleotides each of which consists of
a first segment having a minimum length of about 25
nucleotides and a maximum length of about 100 nucleotides
which segment is at least 90% homologous to a segment of
HTLV-1 nucleic acid, wherein said first segment comprises a
nucleotide sequence selected from the group consisting of
SEQ ID NOS: 42-53; and
a second segment consisting of a nucleotide sequence
which is at least 90% homologous to an oligonucleotide bound
to a solid phase wherein said second segment is not
complementary to HTLV-1 nucleic acid;
and optionally one or more noncomplementary
segments each consisting of a nucleotide sequence that is not
complementary to HTLV-1 nucleic acid;
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