Appeal No. 1997-4277
Application No. 08/290,038
cells formed by viable normal human fetal bone fragments and normal
human fetal spleen grown in juxtaposition.
5. A method for producing a chimeric mouse capable of long term
production, for greater than twenty weeks, of human myeloid cells, B-cells
and lymphoid progenitor cells, said method comprising:
implanting viable normal human fetal spleen and normal human fetal
bone fragments in juxtaposition at a sub-cutaneous site in an
immunocompromised mouse host lacking functional syngeneic B- and T-
cells due to a genetic defect that results in an inability to undergo
germline DNA rearrangement at the loci encoding immunoglobulins and
T-cell antigen receptors;
whereby said tissue forms a hybrid tissue providing long-term
production, for greater than twenty weeks, of human myeloid cells, B-
lineage cells and lymphoid progenitor cells.
18. A method for determining the repertoire of lineages that are able to
develop from a particular human hematopoietic progenitor cell type, said
method comprising:
implanting viable normal human fetal spleen, normal human fetal bone
fragments and normal human fetal thymus tissue in juxtaposition at a
subcutaneous site in an immunocompromised mouse host lacking
functional syngeneic B- and T-cells due to a genetic defect that results in
an inability to undergo germline DNA rearrangement at the loci encoding
immunoglobulins and T-cell antigen receptors;
irradiating said hybrid tissue;
injected HLA mismatched human hematopoietic progenitor cells into
the cavity of said human bone;
maintaining said host, whereby said tissue forms a hybrid tissue
allowing long-term production, of at least twenty weeks, of human
myeloid cells, B-cells and T-cells; and
determining the repertoire of lineages of hematopoietic cells that
develop having the HLA type of said progenitor cells.1
1 We note appellants’ Brief contains the following typographical error “determining
… that having” should be “determining … that develop having.” Compare claim 18,
Paper No. 7, received January 11, 1996.
2
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