Appeal No. 2000-1906
Application No. 09/063,338
1. A method of producing an autologous bioadhesive sealant from a
single whole blood sample, comprising:
forming an inactive platelet rich plasma from said whole blood sample;
dividing said inactive platelet rich plasma into a first and a second portion;
reactivating said first portion of said inactive platelet rich plasma to form a
clot;
triturating said clot to obtain a serum comprising autologous thrombin from
said reactivated first portion; and
mixing said serum with said second portion of said inactive platelet rich
plasma.
17. A method of producing an autologous bioadhesive sealant from a
single whole blood sample, comprising:
forming an inactive platelet rich plasma and an inactive platelet poor
plasma from said whole blood sample;
reactivating said inactive platelet rich plasma to form a clot;
triturating said clot to obtain a serum comprising autologous thrombin; and
mixing said serum with said platelet poor plasma.
29. A method of producing an autologous bioadhesive sealant from a
single whole blood sample, comprising:
forming an inactive platelet poor plasma from said whole blood sample;
dividing said inactive platelet poor plasma into a first and a second portion;
reactivating said first portion of said inactive platelet poor plasma to form a
clot;
triturating said clot to obtain a serum comprising autologous thrombin; and
mixing said serum with said second portion of said inactive platelet poor
plasma.
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Last modified: November 3, 2007