Appeal No. 2000-1906
Application No. 09/063,338
41. A method of producing an autologous bioadhesive sealant from a
single whole blood sample, comprising:
forming an inactive platelet rich plasma and an inactive platelet poor
sample from said whole blood sample;
reactivating said inactive platelet poor plasma to form a clot;
triturating said clot to obtain a serum comprising autologous thrombin; and
mixing said serum with said platelet rich plasma.
53. A method of producing an autologous bioadhesive sealant from a
single whole blood sample, comprising;
forming an inactive platelet rich plasma from said whole blood sample;
dividing said inactive platelet rich plasma into a first and a second portion;
adding human recombinant thromboplastin to said first platelet rich plasma
portion to produce thrombin; and
combining said thrombin with said second portion of said inactive platelet
rich plasma.
The examiner relies on the following references:
Antanavich et al. (Antanavich ‘007) 5,585,007 Dec. 17, 1996
Antanavich et al. (Antanavich ‘662) 5,788,662 Aug. 04, 1998
Hirsch et al. (Hirsh) 5,643,192 Jul. 01, 1997
Cochrum 5,510,102 Apr. 23, 1996
Barrow et al. (Barrow) 5,391,380 Feb. 21, 1995
Marieb, “Human Anatomy and Physiology,” 2nd ed., Benjamin/Cummings
Publishing Co., pp. 594-596 (1992)
Suzuki M et al. (Suzuki), “Clinical application of the fibrin adhesive,”
Otolaryngology, Vol. 56, No. 11, pp. 949-953 (1984)
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