Appeal No. 1997-2374
Application No. 08/064,352
APPENDIX
CLAIMS:
1. A hybridoma secreting monoclonal antibody 83A25 having the identifying
characteristics of ATCC deposit no. HB 10392.
3. A method of detecting murine leukemia virus (MuLV) in a sample or a culture,
comprising:
a) contacting said sample or culture with a monoclonal antibody against a
common epitope that specifically binds monoclonal antibody 83A25, said epitope being
present on the envelope glycoproteins of ecotropic, xenotropic, polytropic, and
amphotropic murine leukemia virus, under conditions such that an immunocomplex can
form between said monoclonal antibody and said envelope glycoprotein epitope;
and
b) detecting the presence of an immunocomplex consisting essentially of said
monoclonal antibody and said envelope glycoprotein.
4. A method for neutralizing murine leukemia virus in vitro, comprising:
reacting murine leukemia virus with a sufficient amount of a monoclonal antibody
against a common epitope that specifically binds monoclonal antibody 83A25, said
epitope being present on the envelope glycoproteins of ecotropic, xenotropic,
polytropic, and amphotropic murine leukemia virus, to form a neutralizing
immunocomplex between said monoclonal antibody and said envelope glycoprotein
epitope.
5. A method of purifying leukemia virus or viral protein comprising attaching antibody
83A25 to a matrix and adsorbing the leukemia virus or viral protein to said attached
antibody, thereafter washing the impurities and then eluting the adsorbed virus or the
viral protein from said matrix and recovering the purified virus or viral protein.
6. The method according to claim 3, wherein said murine leukemia virus is selected
from the group consisting of ecotropic, xenotropic, polytropic, and amphotropic MuLV.
7. A monoclonal antibody against a common epitope on the envelope glycoprotein of
ecotropic, xenotropic, polytropic, and amphotropic murine leukemia virus that
specifically binds monoclonal antibody 83A25.
8. A reagent kit comprising a container having an immunoreactive amount of
monoclonal antibody 83A25 and instructional materials to perform appropriate assays
or tests.
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