Appeal No. 1998-1987
Application No. 07/915,783
said antigenic factors being insoluble in the aqueous
medium;
b) adding a non-ionic detergent to the suspension to
disperse the antigenic, insoluble factor(s);
c) separating the dispersed antigenic, insoluble factors
from the non-ionic detergent; and
d) recovering said dispersed antigenic, insoluble
factor(s).
The examiner relies on the following references:
D’Antonio 4,859,464 Aug. 22, 1989
Ludford et al. (Ludford), ”Babesia argentina, plasmodium vivax and P. falciparum:
Antigenic cross-reactions,” Experimental Parasitology, Vol. 32, pp. 317-326
(1972)
Schmidt-Ullrich et al. (Schmidt-Ullrich), “Plasmodium knowlesi-induced antigens
in membranes of parasitized rhesus monkey erythrocytes,” Proc. Natl. Acad. Sci.,
Vol. 75, No. 10, pp.4949-4953 (1978)
Kilejian, “Stage-specific proteins and glycoproteins of Plasmodium falciparum:
Identification of antigens unique to schizonts and merozoites,” Proc. Natl. Acad.
Sci., Vol. 77, No. 6, pp. 3695-3699 (1980)
Epstein et al. (Epstein), ”Monoclonal antibodies against a specific surface
determinant on malarial (Plasmodium knowlesi) merozoites block erythrocyte
invasion,” Journal of Immunology, Vol. 127, No. 1, pp. 212-217 (1981)
Newbold et al. (Newbold), ”Identification of a schizont- and species- specific
surface glycoprotein on erythrocytes infected with rodent malarias,” Moleculer
and Biochemical Parasitology, Vol. 5, pp. 45-54 (1982)
Howard et al. (Howard), ”Solubilization and immunoprecipitation of 125I-labelled
antigens from Plasmodium knowlesi schizont-infected erythrocytes using non-
ionic, anionic and zwitterionic detergents,” Parasitology, Vol. 88, pp. 27-36 (1984)
Perrin et al. (Perrin), ”Malaria : immunity, vaccination and immunodiagnosis,”
Experientia, Vol. 40, pp. 1343-1350 (1984)
Butcher, “Mechanisms of immunity to malaria and the possibilities of a blood-
stage vaccine: a critical appraisal,” Parasitology, Vol. 98, pp. 315-327 (1989)
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