Appeal No. 1999-0123
Application No. 08/406,239
Claim 1 is illustrative of the subject matter on appeal and is reproduced
below:
1. A method for assaying for the presence of an enzymatically active
hydrolase in a sample, said method comprising:
(a) placing said sample in a test device such that said sample contacts a
first solid support in said test device, said first solid support having a
reporter enzyme in dry form and covalently attached thereto in such a
manner whereby said reporter enzyme is released upon action of said
hydrolase, and such that said sample simultaneously contacts a
second solid support having immobilized thereon in dry form an
indicator, said indicator being one which is susceptible to a
detectable change upon action of said reporter enzyme but not
susceptible to said change in the absence of said sample; and
(b) observing whether said indicator undergoes a detectable change,
said detectable change being an indication of the presence of said
enzymatically active hydrolase in said sample.
The references relied upon by the examiner are:
Goldman et al. (Goldman) 5,120,718 Jun. 9, 1992
Mielke et al. (Mielke) 5,156,954 Oct. 20, 1992
Herrmann et al. (Herrmann) 5,202,233 Apr. 13, 1993
Zherdev, “Method of proteolytic activity determination using bovine serum albumin
conjugate with peroxidase,” Zh. Microbiological Epimeriology Immunobiology, pp.
51-55 (1988)1
1 The examiner relied on an official translation of this foreign document.
2
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