Appeal No. 1999-1271
Application No. 08/467,883
(b) selectively extracting P2 protein from the cell paste to produce a
first supernatant containing said P2 protein substantially free from P1 and P6
protein and a residual precipitate containing P1 and P6 protein;
(c) separating the first supernatant from said residual precipitate;
(d) concentrating the P2 protein in said first supernatant to produce a
second supernatant;
(e) purifying P2 protein in said second supernatant substantially free
from pyrogens, lipopolysaccharides and other impurities solubilized from said
paste by said selective extraction step;
(f) selectively extracting P1 protein from the residual precipitate from
step (b) to produce a third supernatant containing P1 protein and a P6-containing
precipitate;
(g) separating said third supernatant from said P6-containing
precipitate;
(h) concentrating the P1 protein in said third supernatant to produce a
fourth supernatant;
(i) purifying P1 protein in said fourth supernatant substantially free
from pyrogens, lipopolysaccharides, P2 protein and other impurities solubilized in
step (f);
(j) selectively extracting the P6-containing precipitate to produce a P6-
containing supernatant and a first extracted precipitate;
(k) separating said P6-containing supernatant from said first extracted
precipitate;
(l) concentrating the P6 protein in said P6-containing supernatant to
produce a fifth supernatant; and
(m) purifying P6 protein in said fifth supernatant substantially free from
pyrogens, lipopolysaccharides, P1 protein and other impurities solubilized in step
(j).
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