Appeal No. 1997-0798
Application 08/128,020
Claims 22, 29, and 32 are representative of the subject matter on appeal and
read as follows:
22. A kit for immunoassay determination of myocardial necroses in a patient, said
kit comprising an antibody to human cardiac muscle troponin T, having a cross reactivity
to troponin I and other myofibrillar proteins of less than 2% as determined by ELISA and
cross reactivity to human skeletal muscle troponin T of less than 5% as determined by
ELISA, and, in a separate container, a binding partner B for either human cardiac
muscle troponin T or for said antibody, with one of said antibody and said binding
partner being labeled with a determinable group.
29. A method for the immunoassay determination of myocardial necroses in a
patient, wherein detecting cardiac muscle injury can be accomplished for at least 150
hours after occurrence of an infarction, said method comprising:
a) incubating a body fluid sample of the patient with
i) at least one antibody to human cardiac muscle troponin T,
and
ii) a binding partner B for either human cardiac muscle troponin T or the
antibody, wherein either the antibody or the binding partner B is labeled with a
determinable group,
to form an immunological complex containing a determinable group; and
b) determining the determinable group as an indicator of the human cardiac
muscle troponin T in the sample to thereby determine the occurrence of injury to a
cardiac muscle, wherein the at least one antibody to human cardiac muscle troponin T
has a cross-reactivity to human skeletal muscle troponin T which is less than 5% as
determined by ELISA and cross-reactivity to troponin I and other myofibrillar proteins of
less than 2% as determined by ELISA.
32. A conjugate of an antibody to human cardiac muscle troponin T having a
cross-reactivity to human skeletal muscle troponin T which is less than 5% as
determined by ELISA and cross-reactivity to troponin I and other myofibrillar proteins of
less than 2% as determined by ELISA, and a determinable group.
The references relied upon by the examiner are:
Cummins et al. (Cummins) “Cardiac-specific troponin-I radioimmunoassay in the
diagnosis of acute myocardial infarction.” American Heart Journal, Vol. 113, No. 6, pp.
1333-1344, June 1987
Gahlmann et al. (Gahlmann) “Differential Expression of Slow and Fast Skeletal
Muscle Troponin C.” Journal of Molecular Biology, Vol. 201, pp. 379-391, (1988)
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