Appeal No. 1999-2141
Application No. 08/657,164
Claims 8 and 11 are illustrative of the claims on appeal and read as follow:
8. A method for preparing beta taipoxin from taipan snake venom in a single
pass through a high pressure liquid chromatograph, said method comprising:
diluting the taipan snake venom with phosphate buffer saline,
separating a supernatant liquid from the diluted venom, and
fractionating the supernatant liquid on a high pressure liquid chromatograph, using
an ion exchange column and a gradient buffer consisting essentially of (2-amino-
2(hydroxymethyl)propane-1,3-diol)-HCl and water and having a pH in the range of
6.0 - 8.0 to elute a venom fraction consisting essentially of beta taipoxin.
11. A method for separating beta taipoxin from a snake venom fraction which
comprises beta taipoxin together with alpha taipoxin and gamma taipoxin, said method
comprising
fractionating the snake venom fraction on a high pressure liquid chromatograph,
using an ion exchange column and a gradient buffer consisting essentially of (2-amino-2-
(hydroxymethyl)propane-1,3-diol)-HCl and water and having a pH in the range of 6.0 - 8.0
to elute the beta taipoxin separately from the alpha taipoxin and the gamma taipoxin.
The prior art references relied upon by the examiner are:
Haast (Haast ‘762) 4,341,762 Jul. 27, 1982
Haast (Haast ‘902) 4,741,902 May 3, 1988
Fohlman et al. (Fohlman), “Taipoxin, an Extremely Potent Presynaptic Neurotoxin from the
Venom of the Australian Snake Taipan (Oxyuranus s. scutellatus),” Eur. J. Biochem., Vol.
68, pp. 457-469 (1976)
Lind, “Amino-Acid Sequence of the $1 Isosubunit of Taipoxin, and Extremely Potent
Presynaptic Neurotoxin from the Australian Snake Taipan (Oxyuranus s. scutellatus),” Eur.
J. Biochem., Vol. 128, pp. 71-75 (1982)
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