VISSER et al v. HOFVANDER et al - Page 18




          Interference 103,579                                                        

               19.  Potato plant whose genome comprises the antisense                 
               construct as claimed in claim 8.                                       
               20.  Potato tubers whose genome comprises the antisense                
               construct as claimed in claim 8.                                       
               21.  A method for tuber-specific expression of a gene                  
               product in potato, comprising transforming said potato with            
               a DNA molecule comprising an isolated promoter from the                
               potato gene coding for granule-bound starch synthase (GBSS).           
               22.  Antisense construct as claimed in claim 7,                        
               characterized in that the promoter has the sequence                    
               stated in SEQ ID No. 4.                                                
               23.  A method for tuber-specific expression of a gene                  
               product in potato, comprising transforming said potato with            
               a DNA molecule comprising an isolated promotor [sic] from              
               the potato gene coding for granule-bound starch synthase               
               (GBSS), said promoter having the nucleotide sequence stated            
               in SEQ ID No. 4.                                                       
               50.  A method of suppressing amylose formation in potato,              
               wherein the potato is modified by genetic engineering,                 
               which method comprises cultivating a potato containing                 
               in the genome of a tissue of said potato a gene construct              
               comprising a fragment of the potato gene which codes for               
               formation of granule-bound starch synthase (GBSS gene)                 
               inserted in the anti-sense direction, wherein said fragment            
               has the nucleotide sequence of SEQ ID No. 1.                           
               Visser’s claims designated as corresponding to Count 2 are             
          reproduced below:                                                           
               1.  A transgenic potato plant which, as a result of genetic            
               engineering has a genome containing at least one gene                  
               construct containing a full length potato granule-bound                
               starch synthase (PGBSS) cDNA or genomic DNA sequence                   
               coding for PGBSS in reverse orientation in an expression               
               cassette which is functional in potato plants, said gene               
               construct giving rise to tubers containing essentially                 
               amylose free starch; wherein said expression cassette                  
               comprises in the 5'-3' direction of transcription: an                  
               upstream promoter base sequence, a base sequence for                   
                                        -18-                                          





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