Interference 103,579
least one gene construct containing a full length potato
granule-bound starch synthase (PGBSS) cDNA or genomic DNA
sequence coding for PGBSS in reverse orientation in an
expression cassette which is functional in potato plants
comprising in the 5'-3' direction of transcription: an
upstream promoter base sequence, a base sequence for
transcription into mRNA under control of said upstream
promoter base sequence comprising coding and template
strands, and a downstream transcription terminator base
sequence functional in said cell wherein a transformed
cell is obtained; and growing said transformed plant
cell, wherein the coding strand of said base sequence
for transcription comprises an inverted sequence of bases
complementary to a run of bases of PGBSS mRNA, wherein
the transcript of said base sequence for transcription
substantially inhibits the expression of potato granule-
bound starch synthase.
16. The method according to claim 15 wherein said
construct further comprises T-DNA.
17. The method according to claim 15 wherein upsteam
promoter sequence is CaMV35S promoter.
18. The method according to claim 15 wherein upstream
promoter sequence is PGBSS promoter.
19. The method according to claim 15 wherein said
terminator is nopaline synthase terminator.
20. The method according to claim 15 wherein said
terminator is potato granule-bound starch synthase
terminator.
22. The method according to claim 15 wherein the gene
construct was integrated into the potato genome by
transformation with Agrobacterium selected from a group
consisting of Agrobacterium rhizogenes and Agrobacterium
tumefaciens.
23. A homologous construct of the potato plant comprising
a full length potato granule-bound starch synthase (PGBSS)
cDNA or genomic DNA.
24. The homologous construct according to claim 23 wherein
the PGBSS cDNA or genomic DNA is in reverse orientation.
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Last modified: November 3, 2007