Appeal No. 2000-1929
Application No. 08/019,297
Page 8, lines 10-15. The specification also provides the following description of
the other proteins detected by denaturing polyacrylamide gel electrophoresis:
The viral origin of other proteins seen in polyacrylamide gel
electrophoresis of purified virus is more difficult to assess. A p15
protein could be seen after silver staining, but was much weaker
after 35S-methionine perhaps due to the paucity of this amino-acid
in the protein. In the higher MW range, a contamination of the virus
by cellular proteins, either inside or outside the viral envelope, is
likely. A 36K and a 42K protein and a 80K protein were constantly
found to be associated with the purified virus and may represent
the major envelope proteins.
Page 8, lines 21-31.
The specification teaches the use of extracts of viral proteins for
diagnosing AIDS. “The invention further relates to a method of in vitro diagnosis
of LAS or AIDS, which comprises contacting a serum or other biological medium
from a patient to be diagnosed with a viral extract . . . and detecting the
immunological reaction.” Page 10, line 30 to page 11, line 2. The viral extracts
useful in this assay are defined at page 9, lines 1-4: “The invention concerns
more particularly the extracts of said virus as soon as they can be recognized
immunologically by sera of patients afflicted with LAS or AIDS.”
The specification teaches that it is the presence in a viral extract of the
p25 core protein, not the higher molecular weight envelope proteins, that
determines whether the extract is recognized immunologically by patient sera.
See page 9, lines 8-20:
As a matter of fact and except under exceptional circumstances,
sera of diseased patients do not recognize the intact LAV1 virus. . . .
The envelope proteins of the virus appeared as not detectable
immunologically by the patients’ sera. However as soon as the
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