Appeal No. 2001-0121 Page 2
Application No. 08/212,185
(b) determining the amount of binding between the purified
activated receptor recognition factor and the DNA ligand in
the presence of the small molecule; and
(c) comparing the amount of binding in step (b) with the amount
of binding determined in the absence of the small molecule;
wherein the small molecule is identified as a potential drug
having the ability to modulate the binding of the activated
receptor recognition factor to the DNA ligand in vitro when
said comparing indicates a change in the amount of binding
of the activated receptor recognition factor to the DNA
ligand.
103. A method of detecting a phosphorylated receptor recognition factor
comprising:
(a) contacting the phosphorylated receptor recognition factor
with a labeled-antibody specific for the phosphorylated
receptor recognition factor under conditions in which the
labeled-antibody binds to the phosphorylated receptor
recognition factor; and
(b) detecting the labeled-antibody bound to the phosphrylated
receptor recognition factor, wherein said detecting allows the
detection of the phosphorylated receptor recognition factor.
The references relied upon by the examiner are:
Fu et al. (Fu), “ISGF3, the transcriptional activator induced by interferon α,
consists of multiple interacting polypeptide chains,” Proc. Natl. Acad. Sci. USA,
Vol. 87, pp. 8555-8559 (1990)
Decker et al. (Decker), “Two distinct Alpha-Interferon-Dependent signal
transduction pathways may contribute to activation of transcription of the
guanylate-binding protein gene,” Mol. Cell. Bio., Vol. 11, No. 10, pp. 5147-5153
(1991)
The reference relied upon by appellants is:
Darnell, Molecular Cell Biology Fig. 6-2 legend (J. Darnell et al. eds., 2nd ed.,
1990)
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