Appeal No. 2003-0233
Application 09/103,874
33. A pliable soft tissue specimen which has been prepared
in a process comprising the steps of:
(1) treating natural soft tissue obtained from a donor with:
(a) liquid compositions of gradually increasing
concentrations of a polar organic solvent or solvents, until
the last of said liquid compositions contains at least
approximately 25% and at most 75% by volume of said solvent,
or mixture of solvents, the balance being water, the solvent
being selected from a group consisting of aliphatic alcohols
having 1 to 3 carbons and other water miscible polar organic
solvents;
(b) thereafter with a second liquid composition of
aqueous glycerol or of low molecular weight polyethylene
glycol having a molecular weight less than approximately
1000D, containing the glycerol or the low molecular weight
polyethylene glycol, or mixtures thereof being in a
concentration range of approximately 10 to 50% by volume,
said second liquid composition also containing approximately
3 - 20% weight by volume polyethylene glycol of a molecular
weight in the range of 6,000 D to 15,000 D and approximately
2 to 75 units per milliliter heparin of a molecular weight
greater than approximately 3KD;
(2) thereafter briefly immersing the soft tissue in an
aqueous heparin solution, and
(3) thereafter freezing the tissue and lyophilizing the
tissue to dryness.
THE REFERENCES
Baumgartner 4,300,243 Nov. 17, 1981
Noishiki et al. (Noishiki) 4,704,131 Nov. 3, 1987
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