Patent Interference No. 103,925 Page 8
phe leu ser arg val phe thr asn ser leu val phe gly thr ser
110 120
asp arg val tyr glu lys leu lys asp leu glu glu gly ile leu
130
ala leu met arg glu leu glu asp gly thr pro arg ala gly gln
140 150
ile leu lys gln thr tyr asp lys phe asp thr asn met arg ser
160
asp asp ala leu leu lys asn tyr gly leu leu ser cys phe arg
170 180
lys asp leu his lys thr glu thr tyr leu arg val met lys cys
190 191
arg arg phe gly glu ala ser cys ala phe.
41. A method to produce bovine growth hormone from a recombinant DNA vector,
which method comprises:
a) preparing cDNA from RNA isolated from a bovine source which DNA encodes
bovine growth hormone;
b) preparing a first DNA segment from the cDNA prepared in step a);
c) preparing a second DNA segment from a host vector capable of replicating in
a microorganism and containing control sequences for expression;
d) ligating the first DNA segment to the second DNA segment to form an
expression vector effective in expressing the first DNA segment encoding bovine growth
hormone;
e) inserting the expression vector into a microorganism;
f) growing the microorganism in culture under conditions wherein said bovine
growth hormone is produced; and
g) recovering said bovine growth hormone from the culture.
57. A method to produce bovine growth hormone which method comprises recovering
the bovine growth hormone from the microorganism of claim 40.
Ø Claim 13 of Aviv '519 application:
13. A method of producing bovine growth hormone comprising the amino acid
sequence encoded by the coding sequence from nucleotide 87 to nucleotide 659
depicted in Figure 1 comprising extracting RNA from bovine pituitaries, transcribing the
RNA so extracted into DNA, splicing the resulting DNA into a plasmid, inserting the
plasmid containing the spliced DNA into a microorganism, selecting and isolating
microorganism colonies which produce bovine growth hormone and extracting the
bovine growth hormone.
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Last modified: November 3, 2007