Appeal No. 2001-2401
Application 08/277,225
follow:
1. A method of detecting the presence or level of an analyte in a sample, the
method comprising the steps of:
(a) providing a sample containing an analyte;
(b) mixing said sample with a second ligand, which second ligand binds to
said analyte when incubated therewith, so that analyte/second ligand complexes are
formed;
(c) providing a solid phase having bound thereto a first ligand, which first
ligand is characterized by an ability to bind to said second ligand in such a way that,
were said first ligand and analyte exposed simultaneously to unbound second ligand,
said first ligand would compete with said analyte for binding to said second ligand;
(d) contacting the mixture produced in step (a) with said solid phase so that
unbound second ligand in said mixture binds to said first ligand on said solid phase,
said contacting being performed for a time sufficiently limited that substantially no
dissociation of said analyte/second ligand complexes occurs while said mixture is in
contact with said solid phase;
(e) binding a detectable tag to said second ligand either prior to or after step
(a), step (b), step (c), or step (d) so that a portion of said tag is retained on said solid
phase upon formation of said first ligand/second ligand complex; and
(f) detecting said portion of said tag and comparing it to an amount of tag
retained on the column in the presence of a known amount of analyte to determine the
presence or level of said analyte in said sample.
11. A method of detecting the presence or level of an analyte in a sample, the
method comprising steps of:
(a) contacting said sample with:
(i) a solid phase having bound thereto a first ligand that binds said analyte
when incubated therewith; and
(ii) a second ligand that binds said analyte when incubated therewith, the
result of the two contacting steps being that a first ligand/analyte/second ligand complex
is formed on said solid phase[;]
2
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